Proteomics - Pete’s proteomics blog

Disclaimer: The content shared here reflects only my research and perspectives. It is not intended to serve as an official source or formal reference.

UniProt – The Central Hub for Protein Knowledge

For someone who works with protein, you should once in a lifetime search for the protein name to find its function. Doing so, odds are, that your search will land on the…

MS file formats—gotta convert ’em all!

Want to work with MS files yourself but do not have access to licensed software? No worries! These days, a lot of free-to-use proteomics tools (academic use mostly) are now…

Data-dependent acquisition vs. Data-independent acquisition

If you have ever worked with untargeted proteomic results, you would likely encounter the terms data-dependent acquisition (DDA) and data-independent acquisition (DIA).…

Missed cleavage

Sample preparation

In the bottom-up proteomic experiment, proteins are enzymatically digested into peptides prior to mass spectrometry analysis. However, due to the complex structures of…

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